Myeloperoxidase assay in plasma and peritoneal fluid of horses with gastrointestinal disease   总被引：1，自引：0，他引：1       下载免费PDF全文

Sigrid Grulke  Thierry Franck  Monika Gangl  Fabrice Pters  Alexandra Salciccia  Ginette Deby-Dupont    Didier Serteyn 《Canadian journal of veterinary research》2008,72(1):37-42

Gastrointestinal disorders, especially strangulating intestinal obstructions, are still a major cause of illness and death in the horse. Circulating lipopolysaccharides may activate both neutrophils and monocytes. The activated neutrophils release myeloperoxidase (MPO), a specific enzyme with strong oxidative activity. The aim of this study was to evaluate MPO concentrations in the plasma and peritoneal fluid (PF) of horses with colic and to check the hypothesis that these concentrations would be higher in a case of strangulating obstruction than in cases of nonstrangulating disease. By using a specific enzyme-linked immunosorbent assay for equine MPO, we determined the MPO concentrations in horses admitted to a clinic for colic. Horses with nonstrangulating or strangulating obstruction of the large intestine (NSLI or SLI), strangulating obstruction of the small intestine (SSI), or inflammatory bowel disease (IBD) were compared with healthy horses. The horses with SLI, SSI, or IBD had significantly higher MPO levels in plasma and PF than did those in the other 2 groups. The mean plasma level was significantly higher in the horses with NSLI than in the healthy horses. High MPO values in PF indicated necrotic bowel. These results show that neutrophil activation occurs during nonstrangulating and strangulating intestinal obstruction in horses and that the plasma and PF MPO concentrations may be a marker of the severity of the disease.  相似文献   

Canine mast cell tumours: a review of the pathogenesis, clinical features, pathology and treatment     

Welle MM  Bley CR  Howard J  Rüfenacht S 《Veterinary dermatology》2008,19(6):321-339

Mast cells (MCs) are well known for their neoplastic transformation in solitary and multiple cutaneous mast cell tumours (MCTs), as well as visceral and systemic mastocytosis. Dogs have a unique risk of developing cutaneous MCTs, and they account for 7% to 21% of all canine skin tumours. The aetiology of canine MCTs is unknown but is probably multifactorial. This article reviews up-to-date knowledge on the pathogenesis, the clinical presentation, the clinical prognostic factors, the diagnostic workup including clinical staging, cytological findings, histological findings and the various grading systems which have been evaluated based on morphology, the assessment of proliferation markers and other factors such as vessel density. Furthermore, detailed information about current treatment protocols for canine cutaneous MCTs is provided.  相似文献   

Spaying-induced coat changes: the role of gonadotropins, GnRH and GnRH treatment on the hair cycle of female dogs   总被引：1，自引：0，他引：1  

Reichler IM  Welle M  Eckrich C  Sattler U  Barth A  Hubler M  Nett-Mettler CS  Jöchle W  Arnold S 《Veterinary dermatology》2008,19(2):77-87

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Identification and characterization of long non-coding RNAs in porcine granulosa cells exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin     

Monika Ruszkowska  Anna Nynca  Lukasz Paukszto  Agnieszka Sadowska  Sylwia Swigonska  Karina Orlowska  Tomasz Molcan  Jan P.Jastrzebski  Renata E.Ciereszko 《畜牧与生物技术杂志(英文版)》2019,(1)

Background: Long non-coding RNAs(lncRNAs) may regulate gene expression in numerous biological processes including cellular response to xenobiotics.The exposure of living organisms to 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD),a persistent environmental contaminant,results in reproductive defects in many species including pigs.The aims of the study were to identify and characterize lncRNAs in porcine granulosa cells as well as to examine the effects of TCDD on the lncRNA expression profile in the cells.Results: One thousand six hundred sixty-six lncRNAs were identified and characterized in porcine granulosa cells.The identified lncRNAs were found to be shorter than mRNAs.In addition,the number of exons was lower in lncRNAs than in m RNAs and their exons were longer.TCDD affected the expression of 22 lncRNAs(differentially expressed lncRNAs [DELs]; log2 fold change ≥ 1,P-adjusted 0.05) in the examined cells.Potential functions of DELs were indirectly predicted via searching their target cis-and trans-regulated protein-coding genes.The coexpression analysis revealed that DELs may influence the expression of numerous genes,including those involved in cellular response to xenobiotics,dioxin metabolism,endoplasmic reticulum stress and cell proliferation.Aryl hydrocarbon receptor(AhR) and cytochrome P450 1 A1(CYP1 A1) were found among the trans-regulated genes.Conclusions: These findings indicate that the identified lncRNAs may constitute a part of the regulatory mechanism of TCDD action in granulosa cells.To our knowledge,this is the first study describing lncRNAs in porcine granulosa cells as well as TCDD effects on the lncRNA expression profile.These results may trigger new research directions leading to better understanding of molecular processes induced by xenobiotics in the ovary.  相似文献   

Effects of a low dose infusion of racemic and S-ketamine on the nociceptive withdrawal reflex in standing ponies     

Peterbauer C  Larenza PM  Knobloch M  Theurillat R  Thormann W  Mevissen M  Spadavecchia C 《Veterinary anaesthesia and analgesia》2008,35(5):414-423

ObjectiveTo investigate the effect of plasma concentrations obtained by a low dose constant rate infusion (CRI) of racemic ketamine or S-ketamine on the nociceptive withdrawal reflex (NWR) in standing ponies.Study designProspective, blinded, cross-over study.AnimalsSix healthy 5-year-old Shetland ponies.MethodsPonies received either 0.6 mg kg⁻¹ racemic ketamine (group RS) or 0.3 mg kg⁻¹ S-ketamine (group S) intravenously (IV), followed by a CRI of 20 μg kg⁻¹minute⁻¹ racemic ketamine (group RS) or 10 μg kg⁻¹minute⁻¹ S-ketamine (group S) for 59 minutes. The NWR was evoked by transcutaneous electrical stimulation of a peripheral nerve before drug administration, 15 and 45 minutes after the start of the bolus injection and 15 minutes after the end of the CRI. Electromyographic responses were recorded and analysed. Arterial blood was collected before stimulation and plasma concentrations of ketamine and norketamine were measured enantioselectively using capillary electrophoresis. Ponies were video recorded and monitored to assess drug effects on behaviour, heart rate (HR), mean arterial blood pressure (MAP) and respiratory rate.ResultsThe NWR was significantly depressed in group RS at plasma concentrations between 20 and 25 ng mL⁻¹ of each enantiomer. In group S, no significant NWR depression could be observed; plasma concentrations of S-ketamine (9–15 ng mL⁻¹) were lower, compared to S-ketamine concentrations in group RS, although this difference was not statistically significant. Minor changes in behaviour, HR and MAP only occurred within the first 5–10 minutes after bolus drug administration in both groups.ConclusionAntinociceptive activity in standing ponies, demonstrated as a depression of the NWR, could only be detected after treatment with racemic ketamine. S-ketamine may have lacked this effect as a result of lower plasma concentrations, a more rapid metabolism or a lower potency of S-ketamine in Equidae so further investigation is necessary.  相似文献   

Mycobacterium nebraskense infection in a dog in Switzerland with disseminated skin lesions     

Katrin Timm  Monika Welle  Ute Friedel  Danille Gunn‐Moore  Sophie Peterhans 《Veterinary dermatology》2019,30(3):262-e80

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Blepharitis in dogs: a clinical evaluation in 102 dogs     

Christiane Weingart  Barbara Kohn  Martina Siekierski  Roswitha Merle  Monika Linek 《Veterinary dermatology》2019,30(3):222-e69

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Detection of equine X chromosome mosaicism in a mare using an equine X whole chromosome painting probe (WCPP)--a case report   总被引：1，自引：0，他引：1  

Bugno M  Słota E  Pieńkowska-Schelling A  Schelling C 《Acta veterinaria Hungarica》2007,55(2):207-212

An infertile mare with hypoplastic ovaries was subjected to cytogenetic analysis. Fluorescence in situ hybridisation (FISH) using the equine X whole chromosome painting probe (WCPP) was carried out on a chromosome preparation obtained from blood lymphocyte culture. The number of analysed spreads was high (235) and in the X chromosome aneuploidy in mosaic form was diagnosed. The karyotype formula was 63,X / 64,XX / 65,XXX. The ratio of the three lines was 15%, 82% and 3%, respectively. The application of the FISH technique with WCPP is discussed.  相似文献   

Three-Step Test System for the Identification of Novel GABAA Receptor Modulating Food Plants     

Sümeyye Sahin  Volker Eulenburg  Wolfgang Kreis  Carmen Villmann  Monika Pischetsrieder 《Plant foods for human nutrition (Dordrecht, Netherlands)》2016,71(4):355-360

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Effects of light-emitting diode treatments on Brevicoryne brassicae performance mediated by secondary metabolites in Brussels sprouts     

Acharya  Jeewan  Rechner  Ole  Neugart  Susanne  Schreiner  Monika  Poehling  Hans-Michael 《植物病害和植物保护杂志》2016,123(6):321-330

Journal of Plant Diseases and Protection - Although short-wavelength light and especially UV radiation can induce resistance in plants against herbivorous insects, the optimal wavelengths, light...  相似文献